Barnsley's latest signing Lee Collins will go straight into the squad to face Reading at Oakwell.
The 23-year-old defender, who joined on loan from Port Vale on Thursday with a view to a permanent deal, can play anywhere across the back four and manager Keith Hill has confirmed he will "definitely be involved on Saturday".
Goalkeeper David Preece is the club's latest injury victim having been ruled out for the rest of the season with a fractured wrist, so Academy product Lukas Lidakevicius is expected to take his place on the bench.
Left-back Bobby Hassell, midfield pair Jacob Butterfield and David Perkins (all knee), centre-half Rob Edwards (hamstring) and winger Jim O'Brien (groin) remain sidelined.
Striker Frank Nouble, on loan from West Ham, made the first appearance of his second spell at the club last weekend at Southampton and is hoping to retain his place alongside Craig Davies.
Hayden Mullins is in line to make his Reading debut when they travel to Barnsley.
The 32-year-old joined his hometown club on Thursday in a season-long loan deal from Portsmouth and could go straight into Brian McDermott's midfield against the Tykes.
The Royals could also call on Jimmy Kebe's services.
The winger has been troubled by a knee injury in recent weeks but is back in training and McDermott is hopeful he can play some part in their second trip to South Yorkshire of the week following Tuesday's 1-1 draw at Doncaster.
Reading currently occupy an automatic promotion spot but their defensive resources continue to be stretched.
Andy Griffin is back in training from a hamstring injury but this game comes too soon for him and fellow full-back Matthew Connolly is also out due to an ankle problem.